Description:
Abstract Ascorbic acid (Vitamin C) is the important one type of acid for people because of
its quality at suitable content such as increasing the production of collagen, and preventing
the scurvy. Therefore it is necessary to analyze the concentration of ascorbic acid.
Nowadays there is a lot of quantitative analysis to detect ascorbic acid in example
electrochemistry, and high performance liquid chromatography with UV-Visible detector
(or other detector). These techniques are selective, less time consuming and worldwide. In
this work UV-visible spectroscopy was applied as another choice to study the amount of
ascorbic acid because of its simple, less time consuming, and inexpensive. Method
validation of quantitative analysis for ascorbic acid using UV-visible spectroscopy was
reported such as linearity range, precision, and detection limit. These results were shown
that the linearity range was in the range of 0.1-50 μM, %RSD was 0-10%, and detection
limit is 0.02 μM at 265 nm. Moreover the limitation of analysis time was proposed in this
work. The result was presented that the 0-360 minutes analysis time provided the reliable
data. At higher 360 minutes analysis time the absorbance of ascorbic acid was uncertainly.